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potent nos2 inhibitor 1400w 47  (Tocris)


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    Tocris potent nos2 inhibitor 1400w 47
    Figure 4. Decrease in antiinflammatory response and activation of <t>NOS2</t> pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.
    Potent Nos2 Inhibitor 1400w 47, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/potent+nos2+inhibitor+1400w+47/10__1172_slash_jci132987-190-9-15?v=Tocris
    Average 93 stars, based on 69 article reviews
    potent nos2 inhibitor 1400w 47 - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "3D model of harlequin ichthyosis reveals inflammatory therapeutic targets"

    Article Title: 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets

    Journal: Journal of Clinical Investigation

    doi: 10.1172/jci132987

    Figure 4. Decrease in antiinflammatory response and activation of NOS2 pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.
    Figure Legend Snippet: Figure 4. Decrease in antiinflammatory response and activation of NOS2 pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.

    Techniques Used: Activation Assay, In Vitro, Staining

    Figure 5. Inflammation and activation of the STAT1/NOS2 pathway in HI skin. Representative (A) IL-37, (C) IL-36α, (E) IL-36γ, (G) STAT1, (I) p-STAT1, (J) and NOS2 (green channel) and DAPI (blue chan- nel) staining images of control skin and HI patient skin. Arrows indicate granular layer. Associated quantitative analysis of (B) IL-37, (D) IL-36α, (F) IL-36γ, (H) granular layer STAT1, and (K) NOS2 protein expression in control skin and HI patient skin. Each dot represents the mean of relative protein intensity from 3 independent images. n = 3 or 4. Data are represented as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, unpaired t test. Scale bars: 100 μm.
    Figure Legend Snippet: Figure 5. Inflammation and activation of the STAT1/NOS2 pathway in HI skin. Representative (A) IL-37, (C) IL-36α, (E) IL-36γ, (G) STAT1, (I) p-STAT1, (J) and NOS2 (green channel) and DAPI (blue chan- nel) staining images of control skin and HI patient skin. Arrows indicate granular layer. Associated quantitative analysis of (B) IL-37, (D) IL-36α, (F) IL-36γ, (H) granular layer STAT1, and (K) NOS2 protein expression in control skin and HI patient skin. Each dot represents the mean of relative protein intensity from 3 independent images. n = 3 or 4. Data are represented as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, unpaired t test. Scale bars: 100 μm.

    Techniques Used: Activation Assay, Staining, Control, Expressing

    Figure 6. NO release caused epidermal acanthosis and inhibition of NOS2 resulted in normalization of the skin barrier in the HI 3D model. (A) Repre- sentative H&E (bright-field channel) images of in vitro WT and HI 3D models untreated (UT) or treated with SNAP compound. (B) Quantitative analysis of intracellular NO in in vitro WT and HI 3D models, with or without 1400 W inhibitor. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01; ***P ≤ 0.001, 2-way ANOVA with Tukey’s multiple comparisons test. Representative (C) H&E (bright-field channel), (D) Lucifer yellow (LY) (green channel), (E) polar/neutral (red/green channel), (F) GluCer (green channel), and DAPI (blue channel) staining images of in vitro WT and HI 3D models from 3 independent biological replicates. Scale bars: 100 μm.
    Figure Legend Snippet: Figure 6. NO release caused epidermal acanthosis and inhibition of NOS2 resulted in normalization of the skin barrier in the HI 3D model. (A) Repre- sentative H&E (bright-field channel) images of in vitro WT and HI 3D models untreated (UT) or treated with SNAP compound. (B) Quantitative analysis of intracellular NO in in vitro WT and HI 3D models, with or without 1400 W inhibitor. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01; ***P ≤ 0.001, 2-way ANOVA with Tukey’s multiple comparisons test. Representative (C) H&E (bright-field channel), (D) Lucifer yellow (LY) (green channel), (E) polar/neutral (red/green channel), (F) GluCer (green channel), and DAPI (blue channel) staining images of in vitro WT and HI 3D models from 3 independent biological replicates. Scale bars: 100 μm.

    Techniques Used: Inhibition, In Vitro, Staining



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    potent nos2 inhibitor 1400w 47  (Tocris)
    93
    Tocris potent nos2 inhibitor 1400w 47
    Figure 4. Decrease in antiinflammatory response and activation of <t>NOS2</t> pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.
    Potent Nos2 Inhibitor 1400w 47, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/potent+nos2+inhibitor+1400w+47/10__1172_slash_jci132987-190-9-15?v=Tocris
    Average 93 stars, based on 1 article reviews
    potent nos2 inhibitor 1400w 47 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 4. Decrease in antiinflammatory response and activation of NOS2 pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.

    Journal: Journal of Clinical Investigation

    Article Title: 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets

    doi: 10.1172/jci132987

    Figure Lengend Snippet: Figure 4. Decrease in antiinflammatory response and activation of NOS2 pathway in the in vitro ABCA12 KO 3D model. (A) qPCR analysis of IL37 in ABCA12 WT and KO 3D models. n = 3. Data are represented as mean ± SD. NS, P = 0.068, unpaired t test. qPCR analysis of (B) SOCS1 and (C) SOCS3 in ABCA12 WT and KO 2D model cell lysates. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01, unpaired t test. (D) Representative immuno- blot of p-STAT1 (Y701), total STAT1, and GAPDH proteins in untreated (–) or stimulated (+) with IFN-γ ABCA12 WT and KO cell lysates and (E) associated p-STAT1 quantita- tive analysis. n = 4. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test. The p-STAT1 blot was run in parallel, contemporaneously, with total STAT1 and GAPDH blots. (F) Representative NOS2 (green channel) and DAPI (blue channel) staining images of in vitro WT and HI 3D models, and (G) associated quantitative NOS2 analysis. Each dot represents the mean of relative NOS2 intensity from 3 independent images. Scale bars: 100 μm. n = 3. Data are represented as mean ± SD. *P ≤ 0.05, unpaired t test.

    Article Snippet: Daily treatment used 1 μM of the selective and potent NOS2 inhibitor 1400W (47) (1415, Tocris), 100 nM of the JAK1/3 inhibitor tofacitinib (35) (sc-364726, Insight Biotechnology), or 50 μM of the NO-releasing agent S-Nitroso-N-acetyl-DL-penicillamine (SNAP) (51) (sc-200319, Santa Cruz Biotechnology), which was added to the 3D models for 8 days.

    Techniques: Activation Assay, In Vitro, Staining

    Figure 5. Inflammation and activation of the STAT1/NOS2 pathway in HI skin. Representative (A) IL-37, (C) IL-36α, (E) IL-36γ, (G) STAT1, (I) p-STAT1, (J) and NOS2 (green channel) and DAPI (blue chan- nel) staining images of control skin and HI patient skin. Arrows indicate granular layer. Associated quantitative analysis of (B) IL-37, (D) IL-36α, (F) IL-36γ, (H) granular layer STAT1, and (K) NOS2 protein expression in control skin and HI patient skin. Each dot represents the mean of relative protein intensity from 3 independent images. n = 3 or 4. Data are represented as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, unpaired t test. Scale bars: 100 μm.

    Journal: Journal of Clinical Investigation

    Article Title: 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets

    doi: 10.1172/jci132987

    Figure Lengend Snippet: Figure 5. Inflammation and activation of the STAT1/NOS2 pathway in HI skin. Representative (A) IL-37, (C) IL-36α, (E) IL-36γ, (G) STAT1, (I) p-STAT1, (J) and NOS2 (green channel) and DAPI (blue chan- nel) staining images of control skin and HI patient skin. Arrows indicate granular layer. Associated quantitative analysis of (B) IL-37, (D) IL-36α, (F) IL-36γ, (H) granular layer STAT1, and (K) NOS2 protein expression in control skin and HI patient skin. Each dot represents the mean of relative protein intensity from 3 independent images. n = 3 or 4. Data are represented as mean ± SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001, unpaired t test. Scale bars: 100 μm.

    Article Snippet: Daily treatment used 1 μM of the selective and potent NOS2 inhibitor 1400W (47) (1415, Tocris), 100 nM of the JAK1/3 inhibitor tofacitinib (35) (sc-364726, Insight Biotechnology), or 50 μM of the NO-releasing agent S-Nitroso-N-acetyl-DL-penicillamine (SNAP) (51) (sc-200319, Santa Cruz Biotechnology), which was added to the 3D models for 8 days.

    Techniques: Activation Assay, Staining, Control, Expressing

    Figure 6. NO release caused epidermal acanthosis and inhibition of NOS2 resulted in normalization of the skin barrier in the HI 3D model. (A) Repre- sentative H&E (bright-field channel) images of in vitro WT and HI 3D models untreated (UT) or treated with SNAP compound. (B) Quantitative analysis of intracellular NO in in vitro WT and HI 3D models, with or without 1400 W inhibitor. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01; ***P ≤ 0.001, 2-way ANOVA with Tukey’s multiple comparisons test. Representative (C) H&E (bright-field channel), (D) Lucifer yellow (LY) (green channel), (E) polar/neutral (red/green channel), (F) GluCer (green channel), and DAPI (blue channel) staining images of in vitro WT and HI 3D models from 3 independent biological replicates. Scale bars: 100 μm.

    Journal: Journal of Clinical Investigation

    Article Title: 3D model of harlequin ichthyosis reveals inflammatory therapeutic targets

    doi: 10.1172/jci132987

    Figure Lengend Snippet: Figure 6. NO release caused epidermal acanthosis and inhibition of NOS2 resulted in normalization of the skin barrier in the HI 3D model. (A) Repre- sentative H&E (bright-field channel) images of in vitro WT and HI 3D models untreated (UT) or treated with SNAP compound. (B) Quantitative analysis of intracellular NO in in vitro WT and HI 3D models, with or without 1400 W inhibitor. Each dot represents the mean of 3 technical replicates. n = 3. Data are represented as mean ± SD. **P ≤ 0.01; ***P ≤ 0.001, 2-way ANOVA with Tukey’s multiple comparisons test. Representative (C) H&E (bright-field channel), (D) Lucifer yellow (LY) (green channel), (E) polar/neutral (red/green channel), (F) GluCer (green channel), and DAPI (blue channel) staining images of in vitro WT and HI 3D models from 3 independent biological replicates. Scale bars: 100 μm.

    Article Snippet: Daily treatment used 1 μM of the selective and potent NOS2 inhibitor 1400W (47) (1415, Tocris), 100 nM of the JAK1/3 inhibitor tofacitinib (35) (sc-364726, Insight Biotechnology), or 50 μM of the NO-releasing agent S-Nitroso-N-acetyl-DL-penicillamine (SNAP) (51) (sc-200319, Santa Cruz Biotechnology), which was added to the 3D models for 8 days.

    Techniques: Inhibition, In Vitro, Staining